Targeted Sequencing vs. Shotgun Metagenomics

When dealing with complex samples that may contain a mix of various microbial species, such as complex infections, soil, water, or clinical samples in general, the approach chosen for sequencing and analysis can greatly influence the insights obtained.

Targeted Sequencing:


Specificity: Targeted sequencing, often involving markers like the 16S rRNA gene for bacteria or the ITS region for fungi, is highly specific. This means that it can reliably identify the presence of specific microbial taxa even when they are present in low abundances amidst a sea of other microbial DNA. Ripseq offers curated reference databases for targets such as 16S, ITS, rpoB and many more.

Cost-Effective: Since you're focusing only on specific genes or regions, targeted sequencing often requires less sequencing depth, making it more economical compared to shotgun metagenomics, especially for large-scale or routine screening purposes.

Simpler Data Analysis: The bioinformatics pipelines and databases for targeted sequencing (like 16S rRNA gene databases) are well-established and straightforward. The analysis is often more computationally efficient than that of shotgun metagenomics.

Quick Turnaround: Because you're targeting specific regions, the entire process, from sample preparation to data analysis, can be faster. This is particularly valuable in clinical settings where timely identification of pathogens is crucial.

Standardization and Comparability: Given the widespread use of certain markers like the 16S rRNA gene, results from targeted sequencing can be more easily compared across studies or labs.

Shotgun Metagenomics:


Comprehensive: Shotgun metagenomics sequences all the DNA in a sample. This allows for the identification of not just the microbial taxa present but also their functional capabilities, such as metabolic pathways, virulence factors, and resistance genes.

Higher Resolution: While targeted sequencing can generally get you to the genus or sometimes species level, shotgun metagenomics has the potential to differentiate strains, offering a more detailed view of microbial diversity.

Detection of Novel Organisms: Since you're not restricted to known marker genes, shotgun metagenomics can help identify novel or less-studied microorganisms for which targeted primers might not exist.

Functional Insights: Beyond just identification, you can explore the metabolic potential, ecological roles, and interactions of the microbes within the community.

In a complex sample, especially in clinical or public health settings, the primary objective is often to detect and identify potential pathogens. This information guides immediate therapeutic or preventive actions. Targeted sequencing offers a direct and efficient route to achieve this objective. Once pathogens are identified, further in-depth analyses, such as seeking resistance genes, virulence factors, or understanding microbial community dynamics, can be pursued. While shotgun metagenomics offers a rich dataset that can provide these insights in one go, it might be overkill if the immediate need is just pathogen identification. Also, the sheer volume and complexity of data from shotgun metagenomics can delay the time to actionable insights, especially when rapid responses are required.

In summary, while shotgun metagenomics provides a broad and detailed view of complex samples, targeted sequencing is often the method of choice when swift identification of pathogens is the primary concern. The two methods can be complementary, with targeted sequencing serving as a first-line screening tool and shotgun metagenomics offering deeper insights when needed.


Rapid Direct Sequencing Analysis of Clinical Samples using NGS or Sanger. Less than 5 minutes analysis time. No bioinformatics resources needed. Schnelle direkte Sequenzierungsanalyse klinischer Proben mit NGS oder Sanger. Weniger als 5 Minuten Analysezeit. Keine bioinformatischen Ressourcen erforderlich. Analisi rapida di sequenziamento diretto di campioni clinici utilizzando NGS o Sanger. Tempo di analisi inferiore a 5 minuti. Non sono necessarie risorse bioinformatiche. Análisis rápido de secuenciación directa de muestras clínicas mediante NGS o Sanger. Tiempo de análisis de menos de 5 minutos. No se necesitan recursos bioinformáticos. Analyse rapide par séquençage direct d'échantillons cliniques à l'aide de NGS ou de Sanger. Temps d'analyse inférieur à 5 minutes. Aucune ressource bioinformatique nécessaire. Análise rápida de sequenciamento direto de amostras clínicas usando NGS ou Sanger. Tempo de análise inferior a 5 minutos. Não são necessários recursos de bioinformática. NGS 또는 Sanger를 사용한 임상 샘플의 신속한 직접 염기서열 분석. 분석 시간은 5분 미만입니다. 생물정보학 자원이 필요하지 않습니다. 使用 NGS 或 Sanger 对临床样本进行快速直接测序分析。分析时间不到 5 分钟。不需要生物信息学资源。 NGS または Sanger を使用した臨床サンプルの高速直接シーケンス分析。分析時間は 5 分未満です。バイオインフォマティクスのリソースは必要ありません。 Быстрое прямое секвенирование клинических образцов с использованием NGS или Sanger. Время анализа менее 5 минут. Никаких биоинформатических ресурсов не требуется. Hitra analiza neposrednega zaporedja kliničnih vzorcev z uporabo NGS ali Sangerja. Čas analize manj kot 5 minut. Bioinformatični viri niso potrebni. Long-read sequencing, long-read analysis, long-read preprocessor, nanopore, Direct Sequencing analysis, identification, mixed clinical samples, patient, dual loci, HLA, HIV, HPV, bacteria, bacterium, fungi, fungal, virus, Sanger, NGS, Ion, Torrent, Illumina, 16s gene, rpob gene, ITS, ITS2 gene, 16s metagenomics, metagenomic sequencing, amplicon, metagenomic sequencing, dna sequencing, fungal sequencing, isentio, bacterial, sequencing, metagenomic, metagenomics, nucleotide sequence, pathogenomix, pathoseq, pathongs, polymicrobial, sequencing, ripseq, rpob sequencing, second opinion, shotgun, metagenomics, targeted ngs, ngs sequencing, full metagenomic sequencing, amplicon, 16sgene.com, 16smetagenomics.com, 16smetagenomicsequencing.com, 16ssequencingtools.com, ampliconmetagenomicsequencing.com, bacterialsequencing.com, bacterialsequencingtools.com, clinicalmetagenomics.com, dnasequencetools.com, dnasequencingtools.com, fullmetagenomicsequencing.com, fungalsequencing.com, fungalsequencingtools.com, itssequencingtools.com, metagenomics.bio, metagenomics.co, metagenomics.io, metagenomics.us, metagenomicsequencing.com, metagenomix.bio, metagenomix.co, metagenomix.info, metagenomix.io, metagenomix.net, metagenomix.org, metagenomix.us, microbiomeid.com, ngsmetagenomics.com, nucleotidetools.com, optogenetix.com, optogenetix.us, patho-ngs.com, patho-seq.com, PATHOGENOMIX.BIZ, PATHOGENOMIX.CO, PATHOGENOMIX.COM, pathogenomix.info, pathogenomix.io, pathogenomix.net, pathogenomix.org, PATHOGENOMIX.US, pathogenomixnet.com, pathogenomixngs.com, pathoinformatics.com, pathoinformatics.info, pathoinformatics.net, pathoinformatics.org, pathoinformaticsnet.com, pathongs.com, polymicrobialsequencing.com, ripseq.com, ripseq.io, ripseqngs.com, ripsequs.com, rpobgene.com, rpobmetagenomics.com, rpobmetagenomicsequencing.com, rpobsequencingtools.com, secondopinion.io, sequencedecoder.com, shotgunmetagenomics.com, shotgunmetagenomicsequencing.com, targetedngs.com, targetedngssequencing.com